Scientific research in Cell Biology Laboratory is focused on:

· Graft versus host disease pathophysiology
· Juvenile and rheumatoid arthritis pathophysiology
· New methods of non-invasive prenatal diagnosis based on fetal cell and fetal DNA detection in maternal circulation

Research Group and Collaborators include:

Petr Sedlacek, Prof., Dr., Head of Bone Marrow Transplant Unit, 2nd Clinic of Paediatrics, 2nd Medical Faculty, Charles University in Prague, University Hospital Motol, Czech Republic. petr.sedlacek@lfmotol.cuni.cz

Pavla Vavrincova, Dr.,PhD., Rheumatological Out-patient Department, 2nd Clinic of Paediatrics, 2nd Medical Faculty, Charles University in Prague, University Hospital Motol, Czech Republic.

Lucie Sedlackova, Ing., PhD. student, Cell Biology Laboratory, 2nd Medical Faculty, Charles University in Prague, University Hospital Motol, Czech Republic. lucie.sedlackova@lfmotol.cuni.cz

Denisa Zlacka, MSc., PhD. student, Cell Biology Laboratory, 2nd Medical Faculty, Charles University in Prague, University Hospital Motol, Czech Republic. denisa.zlacka@lfmotol.cuni.cz

Nguyen Thi Thu Hien, Dr., PhD. student, Cell Biology Laboratory, 2nd Medical faculty, Charles University in Prague, University Hospital Motol, Czech Republic. hien.nguyen@lfmotol.cuni.cz

For further information about the 2nd Medical Faculty, Charles University in Prague visit the web site - www.lf2.cuni.cz, www.fnmotol.cz

Related EC funded project EUROBANK - www.eurotransplantbank.org

Related Publications

1. Hromadnikova I, Stechova K, Pavla V, Hridelova D, Houbova B, Voslarova S, Nekvasilova H, Vavrinec J. Anti-cyclic citrullinated peptide antibodies in patients with juvenile idiopathic arthritis.
Autoimmunity. 2002 Sep;35(6):397-401.

2. Hromadnikova I, Vavrincova P, Stechova K, Hridelova D.
Anti-keratin antibodies in patients with juvenile idiopathic arthritis.
Clin Exp Rheumatol. 2001 Jul-Aug;19(4):470-3.

3. Stechova K, Vavrincova P, Chudoba D, Frantlova M, Reitzova H, Sosna A, Zimak J, Lednicky L, Dickinson AM, Hromadnikova I. In vitro autoreactivity against skin and synovial cells in patients with juvenile idiopathic and rheumatoid arthritis.
Pathobiology. 2002-2003;70(2):76-82.

4. Stechova K, Vavrincova P, Reitzova H, Chudoba D, Zimak J, Hromadnikova I.
In vitro autoreactivity against skin in rheumatoid arthritis: are peripheral blood mononuclear cells of patients with rheumatoid arthritis able to lyze autologous keratinocytes.
Clin Exp Med. 2001 Jun;1(2):71-4.

5. Jarvis M, Schulz U, Dickinson AM, Sviland L, Jackson G, Konur A, Wang XN, Hromadnikova I, Kolb HJ, Eissner G, Holler E. The detection of apoptosis in a human in vitro skin explant assay for graft versus host reactions.
J Clin Pathol. 2002 Feb;55(2):127-32.

6. Sviland L, Hromadnikova I, Sedlacek P, Cermakova M, Stechova K, Holler E, Eissner G, Schulz U, Kolb HJ, Jackson G, Wang XN, Dickinson AM.
Histological correlation between different centers using the skin explant model to predict graft-versus-host disease following bone marrow transplantation.
Hum Immunol. 2001 Nov;62(11):1277-81.

7. Hromadnikova I, Sedlacek P, Stary J, Cermakova M, Vavrinec J, Stechova K, Dolezalova L, Sviland L, Dickinson AM. An in vitro skin explant assay as a predictive assay for graft versus host disease in a cohort of pediatric transplants.
Pediatr Transplantation 2001; 5:1-12.

8. Dickinson A, Hromadnikova I, Sviland L, Jackson G, Taylor P, Vavrinec J, Sedlacek P, Cermakova M, Stary J, Vitek A, Sajdova J, Proctor S. Use of a Skin Explant Model for Modifying Graft Versus Host Disease Prophylaxis in Adult and Paediatric HLA Identical Allogeneic Stem Cell Transplants.
Bone Marrow Transplantation 1999; 24: 857-863.

9. Hromadnikova I, Sedlacek P, Stary J, Vavrinec J, Cermakova-Frantlova M, Stechova K, Houbova B, Vitek A, Sajdova J, Sviland L, Dickinson AM. A method for modeling skin explants--an in vitro predictive test for graft vs host disease in allogenic hematopoietic cell transplantation.
Cas Lek Cesk 2001; 3: 73-78.

10. Hromadníková I, Cermáková M, Vavrincová P, et al. Monitoring the autoimmune response in patients with rheumatoid arthritis using an in vitro skin explant model.
Osteological Bulletin 2000; 2(5): 70-71.

11. Hromadníková I, Vavrincová P, Vavrinec J, et al. Method of skin explant model - a tool for detection of autoaggression in patients with rheumatoid arthritis.
Czech Rheumatology 1999; 4: 166-174.

12. Hromadníková I, Dickinson AM, Vavrinec J, et al.: An in vitro GvHD prediktive test for allogeneic haematopoietic stem cell transplantation in children- skin explant assay.
Clinical Oncology (Czech journal) 1997; 10:180-183.

13. Hromadníková I, Dickinson AM, et al.: Skin explant model - useful model for studying the pathogenesis of acute GvHD in children Clinical Imunology and Alergology.
(Czech Journal) 1997; 7:5-10.